Uptake and utilization of alanine-1-14C by freshwater algae.

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1969Author
Campbell, David Bennett
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Experiments were conducted x^ith Chlorella pyrenoidosa, Cosmarium
formosulum, and Gloeocapsa sp. to determine their relative heterotrophic
capacity at various conditions. Cultures were incubated in nutrient
media with 0.5 uc DL-alanine-l-^C added at various temperature (10C,
20C, 30C) and illumination (0 ft-c, 2137 ft-c, 8547 ft-c) conditions.
A temperature increase resulted in an increase in alanine uptake in
Ck pyrenoidosa and C. formosulum. Uptake decreased in Gloeocapsa at
20C but increased at 30C. High light intensity and darkness stimulated
uptake in C. pyrenoidosa and C. formosulum and uptake was minimal at
2137 ft-c. Uptake occurred at all illumination conditions with
Gloeocapsa, but was maximum at 2137 ft-c.
Alanine was incorporated into cytoplasmic protein in C. pyrenoidosa
and C.. formosulum but a significant amount was incorporated into protein
in Gloeocapsa only at 2137 ft-c. These species were concluded to be
facultative heterotrophs.