• Login
    View Item 
    •   BEARdocs Home
    • Honors College
    • Theses - Honors College
    • View Item
    •   BEARdocs Home
    • Honors College
    • Theses - Honors College
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Investigating Inhibitory Synergy between Blue Light Irradiation and Antibiotic Treatment of Staphylococcus aureus

    Thumbnail
    View/Open
    Thesis (1.688Mb)
    Honors College Agreement (1.527Mb)
    Access rights
    Worldwide access
    Date
    2013-05-24
    Author
    Fox, Gregg
    Metadata
    Show full item record
    Abstract
    Staphylococcus aureus is a Gram-positive pathogen responsible for significant infections in both the hospital and the community. Rising antibiotic resistance has led to increased infection rates, while the ability of S. aureus to form protective biofilms on medical implants continues to contribute to life-threatening instances of recurrent bacteremia. In an effort to better combat these enhanced defenses, recent research has focused particularly on biofilm disruptors and other alternative treatment methods such as antimicrobial photodynamic therapy (aPDT), all of which have demonstrated synergistic inhibitory effects when combined with antibiotic therapy. This experiment was conducted to search for similar synergistic effects when irradiation with visible blue light, another emerging technique, is coupled with standard antibiotic treatments of oxacillin, neomycin, and ciprofloxacin. During testing, bacterial samples in 24-well plates were exposed to uniform 470nm blue light via an adjustable lightbox. The effects of combined therapy were ascertained through a modified broth microdilution assay with CFUs/mL measured periodically. Data from these tests reveal that the inhibitory effect of blue light alone is relatively dose-independent in the short term, with dosages as low as 5 minutes showing significant inhibition four hours after the beginning of treatment. Combined therapy is even more promising, evincing synergy between blue light and all tested antibiotics in the short-term and between blue light and all but neomycin in the long-term.
    URI
    http://hdl.handle.net/2104/8635
    Department
    Biology.
    Collections
    • Theses - Honors College

    Copyright © Baylor® University All rights reserved. Legal Disclosures.
    Baylor University Waco, Texas 76798 1-800-BAYLOR-U
    Baylor University Libraries | One Bear Place #97148 | Waco, TX 76798-7148 | 254.710.2112 | Contact: libraryquestions@baylor.edu
    If you find any errors in content, please contact librarywebmaster@baylor.edu
    DSpace software copyright © 2002-2016  DuraSpace
    Contact Us | Send Feedback
    TDL
    Theme by 
    Atmire NV
     

     

    Browse

    All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    Login

    Statistics

    View Usage Statistics

    Copyright © Baylor® University All rights reserved. Legal Disclosures.
    Baylor University Waco, Texas 76798 1-800-BAYLOR-U
    Baylor University Libraries | One Bear Place #97148 | Waco, TX 76798-7148 | 254.710.2112 | Contact: libraryquestions@baylor.edu
    If you find any errors in content, please contact librarywebmaster@baylor.edu
    DSpace software copyright © 2002-2016  DuraSpace
    Contact Us | Send Feedback
    TDL
    Theme by 
    Atmire NV