The role of CTR3 in the copper tolerance of nmd mutants of Saccharomyces cerevisiae
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The nonsense-mediated mRNA decay (NMD) pathway is a mechanism by which eukaryotic cells degrade mRNAs with nonsense codons and some natural mRNAs. CTR3 mRNA is a natural mRNA degraded by the pathway and codes for a copper transporter. This study seeks to investigate the regulation of CTR3 by the NMD pathway and the contributions this regulation has on the copper tolerance of nmd mutants of Saccharomyces cerevisiae. This is accomplished through creation of CTR3 gene disruption fragments and transformation of these fragments into S. cerevisiae with an active and an inactive NMD pathway. This creates strains with an active and inactive CTR3. These strains will be grown on both copper-limiting and copper-rich media to assess the copper-tolerant nature of nmd mutants with and without CTR3. At the time of writing, CTR3 gene fragments have been isolated and transformed into yeast strains. Unfortunately, none of the transformants showed CTR3 disruption.