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dc.contributor.advisorKim, Sung Joon
dc.contributor.authorLuper, Elizabeth R.
dc.date.accessioned2014-06-02T18:56:39Z
dc.date.available2014-06-02T18:56:39Z
dc.date.copyright2014
dc.date.issued2014-06-02
dc.identifier.urihttp://hdl.handle.net/2104/9027
dc.description.abstractBiofilms play a major role in the development of drug-resistant, persistent bacterial infections. D-amino acids have been proposed as biofilm inhibitors that prevent biofilm formation by incorporating into the peptidoglycan. We investigated this mechanism of inhibition using SA113, a Staphylococcus aureus clinical isolate that exhibits strong biofilm formation. The dispersive and inhibitory effects of D-tyrosine, D-phenylalanine, and D-proline were measured with a crystal violet biofilm assay. The addition of D-amino acids during or after biofilm formation did not disperse the biofilm or inhibit its formation. To study the proposed D-amino acid incorporation into peptidoglycan, SA113 cells were treated with mutanolysin and then analyzed with liquid chromatography-mass spectrometry (LC-MS). Our initial LC-MS analysis of SA113 grown in the presence and absence of D-amino acids suggested that peptidoglycan composition was not affected by the addition of D-amino acids.en_US
dc.language.isoen_USen_US
dc.rightsBaylor University projects are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. Contact libraryquestions@baylor.edu for inquiries about permission.en_US
dc.subjectBiofilm.en_US
dc.subjectStaphylococcus aureus.en_US
dc.subjectD-amino acids.en_US
dc.subjectPeptidoglycan.en_US
dc.titleThe Effects of D-Amino Acids on Staphylococcus Aureus SA113 Biofilm Formationen_US
dc.typeThesisen_US
dc.rights.accessrightsWorldwide accessen_US
dc.contributor.departmentBiochemistry.en_US
dc.contributor.schoolshonors collegeen_US


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