• Login
    View Item 
    •   BEARdocs Home
    • Graduate School
    • Electronic Theses and Dissertations
    • View Item
    •   BEARdocs Home
    • Graduate School
    • Electronic Theses and Dissertations
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Investigating the role of novel gene -C20orf197.

    View/Open
    WANG-DISSERTATION-2016.pdf (2.619Mb)
    Jingjing_Wang_copyrightavailabilityform.pdf (115.6Kb)
    Access rights
    Worldwide access.
    Access changed 8/16/21.
    Date
    2016-04-05
    Author
    Wang, Jingjing, 1984-
    Metadata
    Show full item record
    Abstract
    The C20orf197 gene which is on chromosome 20 open reading frame 197, was sequenced at least 15 years ago, but even today, it still remains uncharacterised for the public. Meanwhile, over the last few decades it has become apparent that the incidence of acute promyelocytic leukaemia (APL) is increasing. APL is characterised by the accumulation of immature granulocytes, due to these cells being unexpectedly terminated at an early naive differentiation stage. C20orf197 is a novel discovery gene and we have found it has a great effect on granulocyte differentiation. Therefore C20orf197 is a good candidate for a potential novel therapeutic intervention in the treatment of myeloid leukaemia. The NB4 cell line has been used as a model for studies of APL and granulocyte differentiation in vitro. Though the mechanisms underlying APL/granulocyte differentiation have been studied for about three decades, a comprehensive picture of the molecular nature remains incomplete. After several hypotheses have been disproved as shown in supplementary chapter, in this dissertation, based on our own studies, we, in the world wide, for the first time elucidate the function of this novel C20orf197 gene and propose that this gene has an effect on granulocyte differentiation. We first collected and combined all the clues from human tissue, cell lines expression and structure prediction, then we established knockout C20orf197 NB4 cell lines and set up baseline transcriptional profiles of ATRA induced wild type NB4. Then we examined global transcriptional change in the NB4 knockout C20orf197 cell line before and after induction by ATRA. After validation of the transcriptional landscape by cytokines and expression of differentiation markers at single time point and different time points, we found C20orf197 was the contributor for granulocyte differentiation. Thus, our studies provide first-hand information in the world, about the biological role of the uncharacterised novel gene C20orf197 and C20orf197 dependent or independent processes.
    URI
    http://hdl.handle.net/2104/9620
    Collections
    • Electronic Theses and Dissertations
    • Theses/Dissertations - Biomedical Studies

    Copyright © Baylor® University All rights reserved. Legal Disclosures.
    Baylor University Waco, Texas 76798 1-800-BAYLOR-U
    Baylor University Libraries | One Bear Place #97148 | Waco, TX 76798-7148 | 254.710.2112 | Contact: libraryquestions@baylor.edu
    If you find any errors in content, please contact librarywebmaster@baylor.edu
    DSpace software copyright © 2002-2016  DuraSpace
    Contact Us | Send Feedback
    TDL
    Theme by 
    Atmire NV
     

     

    Browse

    All of DSpaceCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    Login

    Statistics

    View Usage Statistics

    Copyright © Baylor® University All rights reserved. Legal Disclosures.
    Baylor University Waco, Texas 76798 1-800-BAYLOR-U
    Baylor University Libraries | One Bear Place #97148 | Waco, TX 76798-7148 | 254.710.2112 | Contact: libraryquestions@baylor.edu
    If you find any errors in content, please contact librarywebmaster@baylor.edu
    DSpace software copyright © 2002-2016  DuraSpace
    Contact Us | Send Feedback
    TDL
    Theme by 
    Atmire NV