Ca2+ Dysregulation Affects Mitochondrial Morphology in Outer Hair Cells

dc.contributor.advisorSimmons, Dwayne
dc.contributor.authorBasoor, Sinchana
dc.contributor.departmentBiology.en_US
dc.date.accessioned2023-05-22T17:54:51Z
dc.date.available2023-05-22T17:54:51Z
dc.date.copyright2023-05-05
dc.date.issued2023-05-22
dc.description.abstract      The normal function of outer hair cells (OHCs) of the cochlea requires a delicate regulation of calcium (Ca2+). Prolonged excessive noise and aging can disrupt this regulation and result in oxidative stress. Oncomodulin (OCM) is the prevalent calcium-binding protein in mammalian cochleae and its presence may moderate the damage caused by injurious stimuli, such as noise. Mitochondria play a central role in oxidative stress and can show signs of stress through changes in their dynamic morphology (size and shape). We hypothesize that a lack of OCM or noise exposure results in distinct changes to the mitochondrial networks of OHCs which may contribute to their dysfunction prior to cell death. To test this, distortion product otoacoustic emissions (DPOAE) assays on wildtype mice and mice lacking OCM were performed to assess hearing ability. Immunofluorescence was performed on cochlear sections to label mitochondria and OHCs for morphology analysis using the Imaris program. Results from these preliminary experiments show that OHCs from mice exposed to noise or lacking OCM exhibit increased hearing thresholds and distinct changes in the mitochondrial morphology of their outer hair cells.en_US
dc.identifier.urihttps://hdl.handle.net/2104/12182
dc.language.isoen_USen_US
dc.rightsBaylor University projects are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. Contact libraryquestions@baylor.edu for inquiries about permission.en_US
dc.rights.accessrightsWorldwide accessen_US
dc.subjectBiologyen_US
dc.subjectHearing Lossen_US
dc.subjectMitochondriaen_US
dc.subjectCalciumen_US
dc.subjectOncomodulinen_US
dc.titleCa2+ Dysregulation Affects Mitochondrial Morphology in Outer Hair Cellsen_US
dc.typeThesisen_US

Files

Original bundle

Now showing 1 - 2 of 2
Loading...
Thumbnail Image
Name:
Thesis Draft 5.4.23 (1).pdf
Size:
2.71 MB
Format:
Adobe Portable Document Format
Description:
Final Thesis
No Thumbnail Available
Name:
Honors form.pdf
Size:
61.07 KB
Format:
Adobe Portable Document Format
Description:
Honors College Agreement

License bundle

Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.87 KB
Format:
Item-specific license agreed upon to submission
Description: