The Longitudinal Comparison of Haplotype Diversity of the Invasive Malaria Vector Anopheles stephensi in Dire Dawa, Ethiopia, 2018 – 2022




Duddu, Sowmya

Access rights

No access - Contact

Journal Title

Journal ISSN

Volume Title



Anopheles stephensi, a species of mosquito native to the Middle East and South Asia, has been seen in the Horn of Africa (HOA) in recent years. Due to the fact that An. stephensi is capable of spreading Plasmodium vivax or falciparum, malaria-causing parasites, its characteristics in the HOA must be studied to provide information for the prevention of population growth. This project aimed to find if there was a difference in the diversity of An. stephensi mosquitos collected in Dire Dawa between the years 2018 and 2022. The conserved Cox1 gene was amplified for each mosquito through PCR and sequenced for further analysis. Sequences representing six of the haplotypes that were observed in Ethiopia from 2018 were compared to the 20 Cox1 sequences from mosquitoes from 2022 to see how the haplotypes presented differed. Haplotype analysis was done on DNAsp and it was seen that the 20 sequences from Dire Dawa, 2022 belonged to three haplotypes and the 23 sequences from Dire Dawa, 2018 belonged to three haplotypes. This infers that the diversity of the Anopheles stephensi mosquitoes between the time of both these collections has not changed.