Cardiac Perfusion Method for Chemically Modifying Kidney Vasculature with Fluorophores

Date

2024

Authors

Cantarella, Jack

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Abstract

Drug linker synthesis is a resource intensive process; therefore, an optimized chemical modification procedure of a target organ is necessary to test newly synthesized drug linkers at reduced costs. We hypothesized that cardiac perfusions of fluorophore attached to N-Hydroxysuccinimide (NHS) ester, which bioconjugates to primary amines, would yield consistent perfusions and fully modified kidney vasculature, while those perfused with unmodifiable linker or saline would not have attached fluorophore psot-perfusion. Anatomically right kidneys were sectioned and imaged using a fluorescent microscope while anatomically left kidneys were macerated, lysed, and analyzed by a microplate reader for total fluorescent intensity. Results demonstrated successful chemical modification of kidney vasculature with NHS-attached fluorophore as images of tissue slides yielded fluorescent vessels post-perfusion and strong fluorescent intensity in NHS lysate solutions.

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Keywords

Chemistry, Biology

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