Cardiac Perfusion Method for Chemically Modifying Kidney Vasculature with Fluorophores

dc.contributor.advisorKane, Robert
dc.contributor.authorCantarella, Jack
dc.contributor.departmentScience Research Fellows.
dc.contributor.otherBaylor University.en
dc.contributor.schoolsHonors College - Honors Programen
dc.date.accessioned2024-05-14T20:00:19Z
dc.date.available2024-05-14T20:00:19Z
dc.date.copyright2024
dc.date.issued2024
dc.description.abstractDrug linker synthesis is a resource intensive process; therefore, an optimized chemical modification procedure of a target organ is necessary to test newly synthesized drug linkers at reduced costs. We hypothesized that cardiac perfusions of fluorophore attached to N-Hydroxysuccinimide (NHS) ester, which bioconjugates to primary amines, would yield consistent perfusions and fully modified kidney vasculature, while those perfused with unmodifiable linker or saline would not have attached fluorophore psot-perfusion. Anatomically right kidneys were sectioned and imaged using a fluorescent microscope while anatomically left kidneys were macerated, lysed, and analyzed by a microplate reader for total fluorescent intensity. Results demonstrated successful chemical modification of kidney vasculature with NHS-attached fluorophore as images of tissue slides yielded fluorescent vessels post-perfusion and strong fluorescent intensity in NHS lysate solutions.
dc.identifier.urihttps://hdl.handle.net/2104/12627
dc.language.isoen_US
dc.rightsBaylor University projects are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. Contact libraryquestions@baylor.edu for inquiries about permission.en
dc.rights.accessrightsNo access - Contact librarywebmaster@baylor.edu
dc.subjectChemistry
dc.subjectBiology
dc.titleCardiac Perfusion Method for Chemically Modifying Kidney Vasculature with Fluorophores
dc.typeThesisen

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