Developing a complex co-culture model for the central nervous system to assess biological interactions within in vitro settings




Kramer, Alec

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Cell culture models are highly utilized in many fields of science. Several models have been created to replicate physiological structures. These models utilize multiple cell types to increase complexity by incorporating physiological responses and cellular communication. Some of these constructed models have been able to replicate structures such as the intestinal lining, alveolar space, and blood-brain barrier. However, there are many more physiological structures which could be replicated through complex co-culture models. The central nervous system (CNS) is comprised of neurons, microglia, astrocytes, and oligodendrocytes. Each of these cell types functions in a specialized role, neurons are involved in cell-to-cell communication, microglia have immune function, astrocytes organize structural components of the CNS, and oligodendrocytes provide support for membrane depolarization. By incorporating these cell types to replicate the CNS would provide a new and innovative method for toxicological and pharmaceutical assessments, and further advance the field of in vitro testing.



Environmental Health