Investigating miR-23a/b Regulation of KDM6A Protein Expression in Epithelial-Mesenchymal Transition Using Breast Cancer Cell Models

dc.contributor.advisorTaube, Joseph
dc.contributor.authorKim, Joelle
dc.contributor.departmentScience Research Fellows.en_US
dc.contributor.otherBaylor Universityen_US
dc.contributor.schoolsHonors Programen_US
dc.date.accessioned2021-05-21T13:09:05Z
dc.date.available2021-05-21T13:09:05Z
dc.date.copyright2021
dc.date.issued2021-05-21
dc.description.abstractTumor progression of epithelial cancers is facilitated by epithelial-mesenchymal transition (EMT) and mesenchymal-epithelial transition (MET), reverse processes that enable invasive cellular behavior. A key regulator of EMT/MET is lysine (K)-specific demethylase 6A (KDM6A), an activator of epithelial gene expression that is abundant in epithelial cell phenotypes. During EMT progression, the level of KDM6A protein expression decreases but there is no change in KDM6A transcript. Understanding the post-transcriptional regulators of KDM6A may reveal new modulators of EMT and potential therapeutic targets. MicroRNAs are short non-coding RNAs that bind mRNA transcripts to repress protein translation. To identify miRNAs that likely target KDM6A, we utilized seven databases as well as in vitro and clinical data to identify miR-23a and miR-23b as putative regulators of KDM6A. We then overexpressed miR-23a/b in breast cancer cells and, unexpectedly, observed no impact on KDM6A protein expression. Further research may explore other means of post-transcriptional regulation of KDM6A, including additional miRNA screening and alternative splicing analysis.en_US
dc.identifier.urihttps://hdl.handle.net/2104/11312
dc.language.isoen_USen_US
dc.rightsBaylor University projects are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. Contact libraryquestions@baylor.edu for inquiries about permission.en_US
dc.rights.accessrightsWorldwide accessen_US
dc.subjectEpithelial-mesenchymal transition.en_US
dc.subjectCancer metastasis.en_US
dc.subjectMicroRNAs.en_US
dc.subjectKDM6A.en_US
dc.subjectEpigenetic modification.en_US
dc.subjectRNA interference.en_US
dc.titleInvestigating miR-23a/b Regulation of KDM6A Protein Expression in Epithelial-Mesenchymal Transition Using Breast Cancer Cell Modelsen_US
dc.typeThesisen_US

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