Genetic suppressor of che-3 serotonin response defects

dc.contributor.advisorLee, Myeongwoo
dc.contributor.authorKirsch, Lauren
dc.contributor.authorChaudhury, Tristin
dc.contributor.authorFarlough, Catera
dc.contributor.authorGamayot, Jannine
dc.contributor.authorRodriguez, Grace
dc.contributor.authorWeinberg, Savannah
dc.contributor.departmentBaylor University. Dept. of Biologyen_US
dc.contributor.otherBaylor University.en_US
dc.contributor.schoolsBaylor University. Dept. of Biologyen_US
dc.date.accessioned2020-04-21T19:15:19Z
dc.date.available2020-04-21T19:15:19Z
dc.date.copyright2019-05-06
dc.date.issued2020-04-21
dc.description.abstractche-3 is a cytosolic dynein heavy chain within Caenorhabditis elegans. This motor protein acts in intraflagellar transport and maintains structural integrity of sensory cilia structures. When mutated at the che-3 genetic locus, the mutants show progressive developmental defects of the chemosensory cilia. The che-3 mutants lose olfactory abilities and are unable to detect and respond to factors in their environment. Therefore, che-3 cannot respond to serotonin stimulation properly. Normally, wild type (N2) C. elegans produce more eggs in the presence of serotonin. Our goal was to test the relationship between serotonin and the olfactory senses within C.elegans. It is not known why che-3 is less responsive to serotonin. To address this, we mutagenized che-3 and found a suppressor that recovers the mutant’s response to serotonin. We created various trials to test a che-3 mutant suppressor that produces more eggs in the presence of serotonin than the wild type. The mutagenized che-3 were bred to yield the recessive F2 generation and placed in serotonin solutions to observe the number of eggs produced. The experiment was repeated many times to yield a consistent outcome. The results that were observed in che-3 mutants, compared to the wild type, produced more eggs in the presence of serotonin when the suppressing mutation occurred. In the future, further testing of che-3 mutants could aid in our understanding of the human response to serotonin and the effects of serotonin uptake within human chemosensory structures in the brain.en_US
dc.identifier.urihttps://hdl.handle.net/2104/10818
dc.language.isoEnglishen_US
dc.rightsBaylor University projects are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. Contact libraryquestions@baylor.edu for inquiries about permission.en_US
dc.rights.accessrightsWorldwide accessen_US
dc.subjectGenetic Suppressor in C. elegansen_US
dc.titleGenetic suppressor of che-3 serotonin response defectsen_US
dc.typePresentationen_US

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