Kim, Sung-Kun, 1968-Park, Ji SooBaylor University.2014-06-022014-06-022014-05-272014-06-02http://hdl.handle.net/2104/9020Since bacterial pathogens are able to develop resistance after exposure to antibiotics, antibiotic resistance is a serious problem in medicine. Resistance to antibiotics occurs by the production of β-lactamases, which cleave the β-lactam bond present in common antibiotics such as penicillin. This study focuses on the overexpression and characterization of the New Delhi metallo-β-lactamase enzyme by conducting studies on the dependence of ionic strength, buffer concentration, and pH on the β-lactamase-catalyzed hydrolysis of Penicillin G. Results showed that NDM-1 was successfully purified. The effect of ionic strength on MOPS buffer showed an initial decrease and then an increase in the kcat/Km, while phosphate buffer showed an increase and then decrease in the kcat/Km value. In the case of buffer effect, MOPS and phosphate buffer showed a strong decrease in kcat/Km as buffer concentration increased. The pH studies showed some scattering data points, suggesting that the protein is unable to endure some acid/base conditions.en-USBaylor University projects are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. Contact libraryquestions@baylor.edu for inquiries about permission.Overexpression and Characterization of Metallo-β-lactamase from Klebsilla pneumoniae: NDM-1ThesisWorldwide access