Lee, MyeongwooPhipps, V.Hartsfield, D.Marsh, T.Shalo, P.Shields, B.Baylor University.2020-04-212020-04-2120192020-04-21https://hdl.handle.net/2104/10821The nematode Caenorhabditis contains hemicentin, which is a protein that aids in gonad development, as well as tissue formation. The him-4 gene, associated with the production of hemicentin, was used to observe the effects of mutagenesis on C. elegans development and to isolate progeny that suppressed it. Five suppressors of him-4 (e1267) were isolated, and its phenotypes were compared to N2 (wild-type) and him-4. Phenotypic analyses revealed there had been a significant amount of suppression of the him-4 mutation. The results of the RNAi indicate some of the suppressors had an increased number of offspring when compared to him-4 mutants by the five suppressors. RNAi pat-3 and ina-1 indicated that some of the suppressors had an increased number of offspring when compared to the him-4 mutants. The overall results of the phenotypic assays, RNAi and hatch rates indicate some degree of suppression and seem to support the possibility of reversing the effects of him-4. Further research into the mechanisms of suppression and gene interaction, could result in significant information could then be applied to the human ortholog of hemicentin.EnglishBaylor University projects are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. Contact libraryquestions@baylor.edu for inquiries about permission.Caenorhabditis elegans.Him-4 gene.Mutation.Suppressor.PresentationWorldwide access