Lee, MyeongwooCroomes, OlineeceBertoluzzi, SienaJohnson, EmilyMarquez, MeaganTajudeen, MariamQiu, ZhonggiangBaylor University.2020-04-212020-04-2120192020-04-21https://hdl.handle.net/2104/10827Emily Johnson, Morenike Tajudeen, Meagan Marquez, Siena Bertoluzzi, Olineece Croomes 7/31/2019 Effect of a cell binding domain mutation in the unc-52 gene of C. elegans Caenorhabditis elegans provides a significant canvas for research due to their sequenced genome, recorded molecular pathways, simple structure and comparative systematic components useful in modeling human diseases. The unc-52 gene in Caenorhabditis elegans encodes a homologouge for the extracellular matrix proteoglycan perlecan. UNC-52 constitutes a structural basement membrane protein which plays an important role in myofilament organization, and a regulator of growth-factor signaling in the body wall muscle cells. To determine the phenotypic effect formed from the presence of unc-52 mutation, we utilized the CRISPR-Cas9 gene-editing technology to mutate the amino acid sequence of the unc-52 gene. We edited the cell-binding domain of unc-52 and produced RGE (arg-gly-glu) from RGD (arg-gly-asp). We injected 52 N2 worms, and successfully generated several homozygous alleles in the C. elegans where the RGD sequences had been transformed to RGE. C. elegans was observed after treatment, and successfully mutated genes produced severely paralyzed uncoordinated worms in the surviving phenotype specimens proceeding CRISPR-cas 9 gene editing. Previous experiments with the unc-52 gene have shown a number of different mutations causing frameshift mutations and nonsense mutations which have been lethal to the organism. The RGD sequence we aim to mutate has been shown to mediate interactions with cell-surface integrins, as well as function in the development of myofilament lattice assembly. A severe phenotypic defect arising from the mutated genes would prove specifically the importance of the RGD sequence in the development of the cytoskeleton and cellular interactions, as well as demonstrate a non-lethal mutant in the unc-52 gene.EnglishBaylor University projects are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. Contact libraryquestions@baylor.edu for inquiries about permission.CRISPR/cas-9C. Elegans mutationUnc-52UNc-52 gene in C. ElegansPresentationWorldwide access