Sim, Cheolho.2016-06-212016-06-212016-052016-04-05May 2016http://hdl.handle.net/2104/9646Development of a transmission-blocking vaccination may provide cost-effective means of preventing the spread of lymphatic filariasis. Here, vaccine candidates were derived from the transcriptomic profiles of two separate developmental life stages of B. pahangi: microfilariae and third-stage larval. Analysis of these differential transcripts provided a detailed overview of expression profiles unique to each developmental stage. Differentially expressed transcripts were scored before the 14 highest ranked transcripts were validated via qRT-PCR. Seven candidate genes were identified as potential targets for transmission-blocking vaccines; two types of Serpins, two types of Cathepsin, Hsp70, Ubiquitin, and Chitinase. Genes selection was limited to secretory or transmembrane proteins in order to increase the proficiency of transmission-blocking vaccine candidates. Finally, we discussed the potential role of each candidate gene in B. pahangi during the midgut infection stage in the mosquito Culex pipiens.application/pdfenCRISPR/Cas9. Culex pipiens. In vitro. Brugia. Brugia pahangi. Transmission blocking vaccine.ThesisWorldwide access2016-06-21