Show simple item record

dc.contributor.advisorBryan Gibbon
dc.contributor.authorNatasha Gambhir
dc.date.accessioned2014-06-02T18:42:37Z
dc.date.available2014-06-02T18:42:37Z
dc.date.copyright2014
dc.date.issued2014-06-02
dc.identifier.urihttp://hdl.handle.net/2104/9007
dc.description.abstractThe aim of the research was to conduct an experiment separating the organelles of the plant, Juniperus virginiana, in a gradient in order to isolate the mitochondria from chloroplasts. Purifying the mitochondria would allow sequencing of the mitochondrial genome of Juniper and learning more about the plant and its history. The challenge of the experiment was to see if the mitochondria and the chloroplast could be separated into different layers in a gradient in order to extract simply the mitochondria. To analyze the organelles found in each layer and locate specific organelles, gel electrophoresis and a western blot with antibodies for mitochondria and chloroplast were used. Several trials with different gradients were conducted including a sucrose gradient, a Percoll gradient and a combined sucrose and Percoll gradient. Each trial alters the procedure in an attempt to further isolate the mitochondria.en_US
dc.language.isoen_USen_US
dc.rightsBaylor University projects are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. Contact libraryquestions@baylor.edu for inquiries about permission.en_US
dc.titleIsolation of Juniper Mitochondriaen_US
dc.typeThesisen_US
dc.rights.accessrightsWorldwide accessen_US
dc.contributor.departmentBiology.en_US
dc.contributor.schoolshonors collegeen_US


Files in this item

Thumbnail
Thumbnail


This item appears in the following Collection(s)

Show simple item record