Fibroblast-Endothelial Cell Interactions in the Heart

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dc.contributor.advisorNeilson, Bill
dc.contributor.authorHoward, Catherine
dc.contributor.departmentBiology.en_US
dc.contributor.otherBaudino, Troy A.en_US
dc.contributor.schoolsHonors College.en_US
dc.date.accessioned2014-06-02T19:03:34Z
dc.date.available2014-06-02T19:03:34Z
dc.date.copyright2014
dc.date.issued2014-06-02
dc.description.abstractAngiogenesis in the heart requires cell-cell communication between fibroblasts and endothelial cells (ECs). We have demonstrated that fibroblast-EC interactions involve the cell surface molecule N-cadherin. In tube formation assays, co-culturing ECs with fibroblasts and N-cadherin blocking antibodies decreased tube formation. Moreover, our studies demonstrated that cell-cell interactions between fibroblasts and ECs result in altered gene and protein expression, specifically with the pro-angiogenic factors IL-6, MCP-1 and VEGF. These changes in expression for IL-6 and MCP-1 require direct cell-cell interactions, while VEGF regulation is through indirect interactions. We have previously shown that fibroblasts and ECs can exchange intracellular material through tight gap junctions both in vitro and in vivo. We hypothesize that small microRNAs (miRNAs) can pass freely through these tight gap junctions. Our initial studies have focused on the pro-angiogenic miRNA, let-7f, which we have shown passes between fibroblasts and ECs and aids in vascular remodeling in the heart. We will continue to characterize the exchange of intracellular materials between cells and examine their roles in the vascular remodeling process following cardiac injury.en_US
dc.identifier.urihttp://hdl.handle.net/2104/9042
dc.language.isoen_USen_US
dc.rightsBaylor University projects are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. Contact libraryquestions@baylor.edu for inquiries about permission.en_US
dc.rights.accessrightsWorldwide accessen_US
dc.subjectCardiac Fibroblasts.en_US
dc.subjectEndothelial Cells.en_US
dc.subjectCardiac Myocytes.en_US
dc.subjectCardiac Remodeling.en_US
dc.subjectAngiogenesis.en_US
dc.subjectExtracellular Matrix (ECM).en_US
dc.subjectmicroRNA.en_US
dc.titleFibroblast-Endothelial Cell Interactions in the Hearten_US
dc.typeThesisen_US

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