High-throughput analytical methods for persistent organic pollutants in high-lipid matrices.

Date

2014-08

Authors

Robinson, Eleanor Marian.

Access rights

Worldwide access.
Access changed 12/11/19.

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Abstract

Persistent organic pollutant (POP) trends and profiles reconstructed from environmental matrices, such as mammal tissues, have provided a wealth of information regarding contaminant behavior and environmental fate. These POP trends and profiles are unique and often irreplaceable due to difficulties in obtaining marine mammal samples. Historically, sample preparation methods for lipid-rich marine mammal matrices require an extraction followed by multiple cleanup and concentration steps. Due to the volatility of select POPs, during sample preparation steps (traditional packed column and GPC cleanup steps followed by a concentration step via nitrogen) there are possibilities for analyte loss and contamination. The goal of sample preparation methods for POPs should seek to reduce the overall number of steps. Selective pressurized liquid extractions (SPLE; an analytical technique that combines PLE with sorbent cleanup) has reduced and/or eliminated the number of cleanup steps associated with organic pollutants extraction and cleanup. SPLE methods for lipid-rich matrices were developed and improved for the trace analysis of multiple classes of organic pollutants (organochlorine pesticides, polychlorinated biphenyls, and polybrominated diphenyl ethers). Analytical methods for lipid-rich matrices, such as marine mammal blubber (Weddell seal Leptonychotes weddelli and bowhead whale blubber Balaena mysticetus) and whale earwax (blue whale Balaenoptera musculus), were developed and emphasized improvements on sample preparation for the analysis of organic pollutants. Marine mammal blubber methods utilizing SPLE followed by GPC were developed (Chapter II; Weddell seal blubber tissue), and improved to eliminate all post-extraction (i.e. GPC) cleanup steps (Chapter III; bowhead whale blubber tissue). An SPLE method for whale earwax with no post-extraction cleanup was also developed (Chapter IV). These methods were applied to environmental samples and utilized to generate irreplaceable data including the first identification of PBDEs in Antarctica’s Weddell seals (Chapter II) as well as in Arctic bowhead whales (Chapter III), and the first identification (Chapter IV) and reconstruction (Chapter V) of contaminant profiles in whale earplugs.

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Keywords

Pesticides., Extraction methods., Sample preparation., Lipid-rich matrices., Polychlorinated biphenyls., Polybrominated dipheyl ethers., Analytical methods.

Citation

Trumble, Stephen J., Robinson, Eleanor M., Noren, Shawn R., Usenko, Sascha, Davis, Jay, Kanatous, Shane B. "Assessment of legacy and emerging persistent organic pollutants in Weddell seal tissue (Leptonychotes weddellii) near McMurdo Sound, Antarctica." Science of the total environment. 439, (2012): 275–283.
Robinson, Eleanor M., Trumble, Stephen J., Subedi, Bikram., Sanders, Rebel, and Usenko, Sascha. "Selective pressurized liquid extraction of pesticides, polychlorinated biphenyls and polybrominated diphenyl ethers in a whale earplug (earwax) : a novel method for analyzing organic contaminants in lipid-rich matrices." Journal of chromatography A. 1319 (2013):14-20.
Trumble, Stephen J., Robinson, Eleanor M., Berman-Kowalewski, Michelle, Potter, Charles W., and Usenko, Sascha. "Blue whale earplug reveals lifetime contaminant exposure and hormone profiles." Proceedings of the National Academy of Science 110, 42 (2013).