Evaluating the Effects of Acute Diesel Exhaust Particle Exposure on P-glycoprotein Function and Expression in the Blood-Brain Barrier

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dc.contributor.advisorBruce, Erica
dc.contributor.authorNunn, Kaitlin
dc.contributor.departmentBiology.en_US
dc.date.accessioned2022-05-20T18:37:45Z
dc.date.available2022-05-20T18:37:45Z
dc.date.copyright2022
dc.date.issued2022-05-20
dc.description.abstractDiesel exhaust particles (DEP) are a major component of air pollution and a source of concern because once inhaled, DEP may reach the brain, contributing to neurodegenerative disorders. One of the blood-brain barrier’s (BBB) main functions is to limit the entry/action of pathogens and xenobiotics like DEP. Specifically, P-glycoprotein (P-gp), a multidrug transporter found in endothelial cells of the BBB, is responsible for the efflux of small molecule drugs and harmful chemicals from the CNS. The goal of this study was to evaluate the effects of acute (24 hr.) DEP exposure on the function and expression of P-gp in the BBB, and in the BBB with microglia in vitro. We used RT-qPCR to measure P-gp expression, immunocytochemistry and fluorescent imaging to qualitatively analyze the presence of P-gp, and a Rhodamine-123 Accumulation Assay to measure the activity of P-gp in the endothelial cells. This work is significant because it is the first to evaluate the effect of DEP on the P-gp of the BBB. The disruption of P-gp and the resulting dysfunction of the BBB may be implicated in air-pollution-related neurodegenerative disorders.en_US
dc.identifier.urihttps://hdl.handle.net/2104/11863
dc.language.isoen_USen_US
dc.rightsBaylor University projects are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. Contact libraryquestions@baylor.edu for inquiries about permission.en_US
dc.rights.accessrightsWorldwide accessen_US
dc.subjectEnvironmental Science.en_US
dc.subjectToxicology.en_US
dc.subjectP-glycoprotein.en_US
dc.subjectDiesel Exhaust Particle.en_US
dc.titleEvaluating the Effects of Acute Diesel Exhaust Particle Exposure on P-glycoprotein Function and Expression in the Blood-Brain Barrieren_US
dc.typeThesisen_US

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