Genetic screening for suppressor mutation in Caenorhabditis elegans odr-3 mutants

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dc.contributor.advisorLee, Myeongwoo
dc.contributor.authorCarroll, Jacqueline
dc.contributor.authorBadra, Anthony
dc.contributor.authorCantu, Analisa
dc.contributor.authorConway, Caitlyn
dc.contributor.authorDiaz, Ashley
dc.contributor.authorGunderson, Annika
dc.contributor.departmentBaylor University. Dept. of Biologyen_US
dc.contributor.otherBaylor University.en_US
dc.contributor.schoolsBaylor University. Dept. of Biologyen_US
dc.date.accessioned2020-04-21T19:16:10Z
dc.date.available2020-04-21T19:16:10Z
dc.date.copyright2019
dc.date.issued2020-04-21
dc.description.abstractA suppressor mutation is a mutation that negates the effects of a different, separate mutation, facilitating a return to the wild-type phenotype. In this experiment, a suppressor mutation screen of a mutation of the odr-3 gene was conducted by observing the egg-laying behaviors of mutagenized Caenorhabditis elegans worms in different chemical stimulants, and comparing the results to the wild-type. A mutant with a successful suppressor mutation of an odr-3 mutation will have similar egg laying behaviors to that of the wild-type (N2). The gene, odr-3, encodes a G protein α subunit that plays an integral role in sensory and olfactory neurons. The role odr-3 plays is vital to olfactory sensation, osmoregulation, and mechanosensory function. In this experiment, two worm lines that each had an odr-3 mutation and a potential suppressor mutation were generated, isolated, and tested: odr-3-JC-17 and odr-3-JC-66. When serotonin was introduced, the odr-3-JC-17 mutant strain showed a similar response in egg laying compared to the wild type, N2. The average number of eggs laid in an hour for each of the N2, odr-3, odr-3-JC-17 and odr-3-JC-66 strains in serotonin solution were 0.18 0.33, 0.21, and 0.44 eggs/hour, respectively. In dopamine solution, odr-3-JC-17 demonstrated similar egg laying behavior to N2. In a dopamine solution, the N2, odr-3, odr-3-JC-17 and odr-3-JC-66 strains laid 1.04, 2.25, 1.29, and 0.40 eggs/hour, respectively. When placed in an imipramine solution, odr-3-JC-17 had a significantly higher value of eggs laid in an hour than the odr-3 mutant. In imipramine solution, the N2, odr-3, odr-3-JC-17, odr-3-JC-66 mutant strains laid 1.56, 1.53, 2.80, and 0.91 eggs/hour, respectively. These results indicate the presence of a suppressor mutation that is at least partially effective in the odr-3-JC-17 mutant strain.en_US
dc.identifier.urihttps://hdl.handle.net/2104/10822
dc.language.isoEnglishen_US
dc.rightsBaylor University projects are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. Contact libraryquestions@baylor.edu for inquiries about permission.en_US
dc.rights.accessrightsWorldwide accessen_US
dc.subjectodr-3.en_US
dc.subjectCaenorhabditis elegans.en_US
dc.subjectC. elegans.en_US
dc.subjectSuppressor mutation.en_US
dc.subjectSerotonin.en_US
dc.titleGenetic screening for suppressor mutation in Caenorhabditis elegans odr-3 mutantsen_US
dc.typePresentationen_US

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