The Mass Spectrometry Analysis of Chlamydomonas Reinhardtii During Nitrogen Starvation

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dc.contributor.advisorSolouki, Touradj, 1963-
dc.contributor.authorLantz, Carter
dc.contributor.departmentUniversity Scholars.en_US
dc.contributor.otherBaylor University.en_US
dc.contributor.schoolsHonors College.en_US
dc.date.accessioned2017-05-23T20:34:05Z
dc.date.available2017-05-23T20:34:05Z
dc.date.copyright2017
dc.date.issued2017-05-23
dc.description.abstractChlamydomonas reinhardtii is a soil microalgae that has been found to produce useful biopharmaceutical and biofuel material. It is known that mutants of Chlamydomonas reinhardtii produce an abundance of lipid compounds during nitrogen starvation. This project aims to identify possible sources of energy for cellular processes and use mass spectrometry and carbon thirteen isotope labeling to characterize the process of lipid production during nitrogen starvation. To conduct this research, the sta6 mutant of Chlamydomonas reinhardtii was first grown in unlabeled acetate for 120 hours followed by nitrogen starvation in [1-13C] labeled acetate for 72 hours. Mass spectrometry analysis of algal headspace at 24, 48, and 72 hours post-starvation shows that Chlamydomonas reinhardtii utilizes the labeled acetate to obtain carbons from outside the cell and incorporate them into fatty acid tails. Moreover, mass spectrometry characterization of post-starved algal contents revealed unique isotopic distributions for observed diacylglycerols and triacylglycerols. Comparisons of results from mass spectrometry analysis with published transcriptome and pathway analyses allows the identification of key enzymes involved in driving the lipid production during nitrogen starvation.en_US
dc.identifier.urihttp://hdl.handle.net/2104/9954
dc.language.isoen_USen_US
dc.rightsBaylor University projects are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. Contact libraryquestions@baylor.edu for inquiries about permission.en_US
dc.rights.accessrightsWorldwide access.en_US
dc.rights.accessrightsAccess changed 8/20/19.
dc.subjectAnalytical Chemistry.en_US
dc.titleThe Mass Spectrometry Analysis of Chlamydomonas Reinhardtii During Nitrogen Starvationen_US
dc.typeThesisen_US

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