Induced Mutation in Caenorhabditis elegans Causes Dopamine Resistance

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dc.contributor.advisorLee, Myeongwoo.
dc.contributor.authorWalker, Brody
dc.contributor.authorGrewal, Amanpreet Singh
dc.contributor.authorGrayson, Nicholas Kallas
dc.contributor.authorHarris, Luke Reed
dc.contributor.authorDiokpa, Chijindu
dc.contributor.authorAceves, Tatiana
dc.contributor.schoolsBaylor University. Dept. of Biology.en_US
dc.date.accessioned2018-08-06T14:55:20Z
dc.date.available2018-08-06T14:55:20Z
dc.date.copyright2018-05
dc.date.issued2018-08-06
dc.description.abstractIn humans, drug addiction is linked to varying dependencies of dopamine levels in the brain. The neurotransmitter is involved in many behavioral mechanisms in animals and mediates the reward pathway. In C. elegans, one of the effects of dopamine is to inhibit motor neuron activity and create a basal slowing response in the N2 (wild types). As a result, egg-laying in wild types is inhibited. To induce egg laying, mutagenized C. elegans were created with EMS (ethyl methansulfonate) to potentially produce a dopamine resistant mutation. After mutagenesis, 334 nematodes were isolated and screened for egg laying behavior. Both wild type and mutant nematodes were exposed to M9 (control), dopamine, and L-dopa (dopamine precursor) solutions. After 1 hour of incubation in these conditions, quantitative analysis was performed to assess the amount of eggs produced. Using an ANOVA test, statistical significance (p<0.001) was found between the wild type and mutant groups both exposed to dopamine. This implies there has been an induced dopamine resistance. The precursor to dopamine, L-dopa, showed similar effects with regard to egg laying behavior. When the dopamine pathway in C. elegans is known, the gene or group of genes implicated for dopamine resistance can be determined. Once identified, a homologous gene in humans could be located and studied for similar drug-resistant effects. The knowledge gained from this research has implications in the fields of gene therapy and drug abuse.en_US
dc.identifier.urihttp://hdl.handle.net/2104/10400
dc.language.isoen_USen_US
dc.rights.accessrightsWorldwide accessen_US
dc.subjectBiologyen_US
dc.subjectDopamineen_US
dc.subjectC. elegansen_US
dc.subjectCaenorhabditis elegansen_US
dc.subjectEMSen_US
dc.subjectInduced mutationen_US
dc.titleInduced Mutation in Caenorhabditis elegans Causes Dopamine Resistanceen_US
dc.typePresentationen_US

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