Isolation of the him-4 suppressor mutation which rescues the vitality of C. elegans

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dc.contributor.advisorLee, Myeongwoo
dc.contributor.authorPhipps, V.
dc.contributor.authorHartsfield, D.
dc.contributor.authorMarsh, T.
dc.contributor.authorShalo, P.
dc.contributor.authorShields, B.
dc.contributor.departmentBaylor University. Dept. of Biologyen_US
dc.contributor.otherBaylor University.en_US
dc.contributor.schoolsBaylor University. Dept. of Biologyen_US
dc.date.accessioned2020-04-21T19:15:58Z
dc.date.available2020-04-21T19:15:58Z
dc.date.copyright2019
dc.date.issued2020-04-21
dc.description.abstractThe nematode Caenorhabditis contains hemicentin, which is a protein that aids in gonad development, as well as tissue formation. The him-4 gene, associated with the production of hemicentin, was used to observe the effects of mutagenesis on C. elegans development and to isolate progeny that suppressed it. Five suppressors of him-4 (e1267) were isolated, and its phenotypes were compared to N2 (wild-type) and him-4. Phenotypic analyses revealed there had been a significant amount of suppression of the him-4 mutation. The results of the RNAi indicate some of the suppressors had an increased number of offspring when compared to him-4 mutants by the five suppressors. RNAi pat-3 and ina-1 indicated that some of the suppressors had an increased number of offspring when compared to the him-4 mutants. The overall results of the phenotypic assays, RNAi and hatch rates indicate some degree of suppression and seem to support the possibility of reversing the effects of him-4. Further research into the mechanisms of suppression and gene interaction, could result in significant information could then be applied to the human ortholog of hemicentin.en_US
dc.identifier.urihttps://hdl.handle.net/2104/10821
dc.language.isoEnglishen_US
dc.rightsBaylor University projects are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. Contact libraryquestions@baylor.edu for inquiries about permission.en_US
dc.rights.accessrightsWorldwide accessen_US
dc.subjectCaenorhabditis elegans.en_US
dc.subjectHim-4 gene.en_US
dc.subjectMutation.en_US
dc.subjectSuppressor.en_US
dc.titleIsolation of the him-4 suppressor mutation which rescues the vitality of C. elegansen_US
dc.typePresentationen_US

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