Investigating the mixture effects of polycyclic aromatic hydrocarbons through in vitro toxicity assessments using clone-9 liver cells.

Gaskill, Stacey Joanne, 1989-

Investigating the mixture effects of polycyclic aromatic hydrocarbons through in vitro toxicity assessments using clone-9 liver cells.

dc.contributor.advisor	Bruce, Erica Dawn.
dc.creator	Gaskill, Stacey Joanne, 1989-
dc.date.accessioned	2015-03-18T16:19:57Z
dc.date.available	2015-03-18T16:19:57Z
dc.date.created	2014-12
dc.date.issued	2014-11-05
dc.date.submitted	December 2014
dc.date.updated	2015-03-18T16:19:58Z
dc.description.abstract	The Environmental Protection Agency is reevaluating current methods for assessing the toxicity of polycyclic aromatic hydrocarbons (PAHs), including the assumption of toxic additivity in mixtures. This study was aimed at testing mixture interactions using select PAH congeners through in vitro cell culture experimentation, and modeling the toxicity using quantitative structure-activity relationships (QSAR). Clone-9 rat liver cells were used to analyze cellular proliferation, viability, and genotoxicity of 15 PAHs in single doses and in binary mixtures. Tests revealed that many mixtures have non-additive toxicity, but display varying mixture effects depending on the mixture composition. QSARs were developed using viability data to predict toxic activity both in single PAH congeners and in binary mixtures. Effective concentrations inhibiting 50% of the cell populations were successfully modelled, with r^2= 0.90, 0.99 and 0.84, respectively. Our findings suggest that PAH mixtures have complex interactions, and continued mixture research will strengthen toxicological assessments of PAHs.
dc.format.mimetype	application/pdf
dc.identifier.uri	http://hdl.handle.net/2104/9242
dc.language.iso	en
dc.rights.accessrights	Worldwide access.	en_US
dc.rights.accessrights	Access changed 5/23/17.
dc.subject	Polycyclic aromatic hydrocarbons. PAH. Binary mixtures. Mixtures. Quantitative structure activity relationships. QSAR. In vitro. Clone-9 cells.
dc.title	Investigating the mixture effects of polycyclic aromatic hydrocarbons through in vitro toxicity assessments using clone-9 liver cells.
dc.type	Thesis
dc.type.material	text
local.embargo.lift	2016-12-01
local.embargo.terms	2016-12-01
thesis.degree.department	Baylor University. Dept. of Environmental Science.
thesis.degree.grantor	Baylor University
thesis.degree.level	Masters
thesis.degree.name	M.S.