Biological responses from contaminants accumulated in seafood using an in vitro human intestinal and liver co-culture.

Sutherland, Grace E., 1995-

Biological responses from contaminants accumulated in seafood using an in vitro human intestinal and liver co-culture.

dc.contributor.advisor	Lavado, Ramon.
dc.creator	Sutherland, Grace E., 1995-
dc.creator.orcid	0000-0002-6532-9152
dc.date.accessioned	2019-12-04T15:57:08Z
dc.date.available	2019-12-04T15:57:08Z
dc.date.created	2019-08
dc.date.issued	2019-06-10
dc.date.submitted	August 2019
dc.date.updated	2019-12-04T15:57:08Z
dc.description.abstract	In vitro bioassays have been useful in predicting mechanisms of toxicity; however, conventional cell-based assays grown in monolayers are unavoidably poor models for human tissues due to the lack of complexity and physiological interplay observed in vivo. To address these limitations, the present study utilizes a combination of human intestinal and hepatic cells in a co-culture model. The purposes of these projects are to evaluate the differences between mono- and co-culture systems related to cytotoxicity and enzyme activity, and apply the co-culture model in the screening of seafood samples collected from the Galveston Bay. It was observed that the co-culture model had greater antioxidant enzyme activity compared to that of the monoculture, suggesting that hepatocytes grown in co-culture may be better suited to facilitate the expression of enzymes in response to xenobiotic metabolism in intestinal cells. This emphasizes the importance of adequate model selection to facilitate assessment of risk.
dc.format.mimetype	application/pdf
dc.identifier.uri	https://hdl.handle.net/2104/10765
dc.language.iso	en
dc.rights.accessrights	Worldwide access
dc.subject	Co-culture. Caco-2. HepaRG. Cytotoxicity. Antioxidant enzymes. Galveston Bay. Red Drum. Black Drum. Spotted Trout. Oyster.
dc.title	Biological responses from contaminants accumulated in seafood using an in vitro human intestinal and liver co-culture.
dc.type	Thesis
dc.type.material	text
thesis.degree.department	Baylor University. Dept. of Environmental Science.
thesis.degree.grantor	Baylor University
thesis.degree.level	Masters
thesis.degree.name	M.S.