Analysis of the microfilariae-specific transcriptome of Brugia pahangi during mosquito infection.

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dc.contributor.advisorSim, Cheolho.
dc.creatorCotten, Michael A., 1992-
dc.date.accessioned2016-06-21T15:46:39Z
dc.date.available2016-06-21T15:46:39Z
dc.date.created2016-05
dc.date.issued2016-04-05
dc.date.submittedMay 2016
dc.date.updated2016-06-21T15:46:39Z
dc.description.abstractDevelopment of a transmission-blocking vaccination may provide cost-effective means of preventing the spread of lymphatic filariasis. Here, vaccine candidates were derived from the transcriptomic profiles of two separate developmental life stages of B. pahangi: microfilariae and third-stage larval. Analysis of these differential transcripts provided a detailed overview of expression profiles unique to each developmental stage. Differentially expressed transcripts were scored before the 14 highest ranked transcripts were validated via qRT-PCR. Seven candidate genes were identified as potential targets for transmission-blocking vaccines; two types of Serpins, two types of Cathepsin, Hsp70, Ubiquitin, and Chitinase. Genes selection was limited to secretory or transmembrane proteins in order to increase the proficiency of transmission-blocking vaccine candidates. Finally, we discussed the potential role of each candidate gene in B. pahangi during the midgut infection stage in the mosquito Culex pipiens.
dc.format.mimetypeapplication/pdf
dc.identifier.urihttp://hdl.handle.net/2104/9646
dc.language.isoen
dc.rights.accessrightsWorldwide access
dc.subjectCRISPR/Cas9. Culex pipiens. In vitro. Brugia. Brugia pahangi. Transmission blocking vaccine.
dc.titleAnalysis of the microfilariae-specific transcriptome of Brugia pahangi during mosquito infection.
dc.typeThesis
dc.type.materialtext
thesis.degree.departmentBaylor University. Dept. of Biology.
thesis.degree.grantorBaylor University
thesis.degree.levelMasters
thesis.degree.nameM.S.

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